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A spatiotemporal study of bacterial community profiles associated with Atlantic bluefin tuna larvae, Thunnus thynnus L., in three Mediterranean hatcheries ArchiMer
Gatesoupe, Joel; Coves, Denis; Ortega, Aurelio; Papandroulakis, Nikos; Vadstein, Olav; De La Gandara, Fernando.
During the first 2 years of larval rearing trials with Atlantic bluefin tuna, survival was a challenging issue. As bacterial colonization of the gut has been shown to play a key role for other species, we studied the profiles of the microbiota associated with individual larvae at different stages in three distant hatcheries. The Bacterial Community Profile (BCP) was quantified based on PCR-DGGE analyses of partial amplicons from 16S rDNA. Considerable individual variability in BCP was observed before onset of feeding, and the BCP did not show regular fluctuation during ontogenesis. Microalgae were added to the rearing tanks in two of the three hatcheries, but it was not possible to distinguish the effect of location from the effect of algal addition on...
Tipo: Text Palavras-chave: Larval rearing; Bacterial diversity; DGGE; Thunnus thynnus.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00075/18648/16197.pdf
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Microbiology and immunology of fish larvae ArchiMer
Vadstein, Olav; Bergh, Oivind; Gatesoupe, Joel; Galindo-villegas, Jorge; Mulero, Victoriano; Picchietti, Simona; Scapigliati, Giuseppe; Makridis, Pavlos; Olsen, Yngvar; Dierckens, Kristof; Defoirdt, Tom; Boon, Nico; De Schryver, Peter; Bossier, Peter.
For most marine aquaculture species, one of the main bottlenecks is the stable production of high quality juveniles. The high and unpredictable mortality in the first weeks after hatching of marine fish larvae remains a challenging problem that needs to be solved. The severity of the problem differs between species, but cannot be considered adequately solved for any species. Both scientific evidence and experience in hatcheries for a variety of fish, shrimp and shellfish species are accumulating as support for the hypothesis that detrimental fish-microbe interactions are the cause of these problems. Host-microbe interactions in reared fish are still poorly understood, except for a few pathogens, and empirical data of the quality required to test this...
Tipo: Text Palavras-chave: Host-microbe interaction; Mast cell; Microbial ecology; Piscidin; Quorum sensing.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00144/25510/23654.pdf
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